Plasmid

Part:BBa_J100284:Design

Designed by: Zachary Shaver   Group: Campbell M Lab   (2016-07-06)


JC184d5 with Mutagenesis Cassette Removed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1525
    Illegal NotI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1407
    Illegal BamHI site found at 2194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1059


Design Notes

All of the JC184d5 plasmid except the mutagenesis cassette was amplified using Gotaq Master Mix. The sequence was simply digested with the Not1 HF restriction enzyme and the two ends of the linearized plasmid were ligated together with NEB rapid ligase.


Source

This part is a modified form of the JC184d5 plasmid that Davidson College received from Liu et al. (Harvard PACE group).

References